anti il 17a primary antibody  (Boster Bio)

Journal: Scientific Reports

Article Title: Osthol ameliorates obesity-associated lipid metabolic disorders by inhibiting ADRA1D-dependent Th17 cell differentiation

doi: 10.1038/s41598-025-20719-x

Figure Lengend Snippet: OST Attenuates Systemic Inflammation and Downregulates Th17-Related Factor Expression. ( A ) KEGG pathway enrichment analysis of downstream targets of OST. This figure is based on data from KEGG (Kyoto Encyclopedia of Genes and Genomes) at https://www.kegg.jp/ ; © Kanehisa Laboratories. ( B ) Flow cytometry was used to evaluate the proportion of CD4⁺IL-17A⁺ cells in the spleen. ( C ) Immunohistochemical staining of liver tissues was performed to assess IL-17A expression. ( D ) Immunofluorescence staining was conducted to detect CD4⁺RORγt⁺ double-positive cells in liver sections. ( E ) Serum levels of IL-6, IL-1β, TNF-α, and IL-17A were measured using ELISA. n = 6. **** P < 0.0001. One-way ANOVA followed by Tukey’s post hoc test was used for multiple group comparisons.

Article Snippet: Sections were blocked for 30–60 min at room temperature with 5% bovine serum albumin (BSA; A9647, Sigma-Aldrich), followed by overnight incubation at 4 °C with anti-IL-17A primary antibody (1:100, HY- P81114 , MedChemExpress).

Techniques: Expressing, Flow Cytometry, Immunohistochemical staining, Staining, Immunofluorescence, Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Osthol ameliorates obesity-associated lipid metabolic disorders by inhibiting ADRA1D-dependent Th17 cell differentiation

doi: 10.1038/s41598-025-20719-x

Figure Lengend Snippet: OST Inhibits Th17 Cell Differentiation in Vitro. ( A ) Flow cytometry was used to assess the proportion of CD4⁺IL-17A⁺ cells in a Th17-polarized cell model treated with different doses of OST. ( B ) RT-qPCR was performed to evaluate mRNA expression levels of RORγt and IL-17A. ( C ) Western blot was performed to evaluate protein expression levels of RORγt, IL-17A, IL-17RA, TRAF6, and Act1. ( D ) ELISA was used to quantify the secretion of IL-6, IL-1β, TNF-α, IL-10, and TGF-β in the culture supernatants. n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. One-way ANOVA followed by Tukey’s post hoc test was used for multiple group comparisons.

Article Snippet: Sections were blocked for 30–60 min at room temperature with 5% bovine serum albumin (BSA; A9647, Sigma-Aldrich), followed by overnight incubation at 4 °C with anti-IL-17A primary antibody (1:100, HY- P81114 , MedChemExpress).

Techniques: Cell Differentiation, In Vitro, Flow Cytometry, Quantitative RT-PCR, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Osthol ameliorates obesity-associated lipid metabolic disorders by inhibiting ADRA1D-dependent Th17 cell differentiation

doi: 10.1038/s41598-025-20719-x

Figure Lengend Snippet: OST Suppresses Th17 Differentiation via an ADRA1D-Dependent Mechanism. ( A ) Volcano plot of differentially expressed genes (DEGs) in the GSE110729 obesity dataset. ( B ) Venn diagram showing the intersection between OST’s potential targets and DEGs from the GSE110729 dataset. ( C ) RT-qPCR was performed to assess the expression of ADRA1D, IL-17A, and RORγt in Th17-polarized CD4⁺ T cells with or without ADRA1D overexpression. ( D ) Western blot was conducted to evaluate the protein expression of ADRA1D, IL-17A, RORγt, IL-17RA, TRAF6, and Act1. ( E ) ELISA was used to measure the levels of IL-6, IL-1β, TNF-α, IL-10, and TGF-β in the culture supernatants. n = 3. *** P < 0.001, **** P < 0.0001. One-way ANOVA followed by Tukey’s post hoc test was used for multiple group comparisons.

Article Snippet: Sections were blocked for 30–60 min at room temperature with 5% bovine serum albumin (BSA; A9647, Sigma-Aldrich), followed by overnight incubation at 4 °C with anti-IL-17A primary antibody (1:100, HY- P81114 , MedChemExpress).

Techniques: Quantitative RT-PCR, Expressing, Over Expression, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Osthol ameliorates obesity-associated lipid metabolic disorders by inhibiting ADRA1D-dependent Th17 cell differentiation

doi: 10.1038/s41598-025-20719-x

Figure Lengend Snippet: ADRA1D Overexpression Attenuates the Metabolic and Immunomodulatory Effects of OST in Vivo. ( A ) Body weight changes were monitored in HFD-fed mice treated with OST. (B) Weights of adipose tissue and liver were measured to assess the effect on organ hypertrophy. ( C ) Serum levels of TG, TC, FFA, ALT, and AST were evaluated by biochemical assays. ( D ) Flow cytometry was performed to assess the proportion of CD4⁺IL-17A⁺ cells in the spleen. ( E ) Immunofluorescence staining of liver tissue was used to detect CD4⁺RORγt⁺ double-positive cell infiltration. ( F ) Immunohistochemistry was performed to evaluate hepatic IL-17A protein expression. ( G ) Western blotting was conducted to assess ADRA1D protein expression in liver tissue. ( H ) Western blot was used to detect the phosphorylation levels of ERK1/2 and PI3K in liver tissue. ( I ) H&E staining of adipose tissue was used to assess adipocyte morphology. (J) Oil red O staining of liver sections was performed to evaluate hepatic lipid deposition. n = 6. ** P < 0.01, **** P < 0.0001. For three or more groups, one-way or two-way analysis of variance (ANOVA) was applied, followed by Tukey’s post hoc test. P < 0.05 was considered statistically significant.

Article Snippet: Sections were blocked for 30–60 min at room temperature with 5% bovine serum albumin (BSA; A9647, Sigma-Aldrich), followed by overnight incubation at 4 °C with anti-IL-17A primary antibody (1:100, HY- P81114 , MedChemExpress).

Techniques: Over Expression, In Vivo, Flow Cytometry, Immunofluorescence, Staining, Immunohistochemistry, Expressing, Western Blot, Phospho-proteomics

Journal: Turkish Journal of Biology

Article Title: tsRNA-15797-modified BMSC-derived exosomes mediate LFNG to induce angiogenesis in osteonecrosis of the femoral head

doi: 10.55730/1300-0152.2654

Figure Lengend Snippet: Angiogenesis-related tsRNA-15797 is low expressed in plasma exosomes of ONFH patients. A, Representative electron micrograph of exosomes (scale bars = 100 nm). B, The NTA was used to measure the size, distribution, and content of plasma exosomes. Y-axis: the content of exosomes/ml. C, Western blot analysis showing the presence of CD9 and CD63 in exosomes derived from the BMSCs. D, The expression of tsRNA in exosomes was determined by q-PCR. E, CCK8 was used to detect the effect of tsRNA on cell viability of HUVECs. F, Effect of exosomes derived from tsRNA-15797 mimics on tube formation ability of HUVECs. *p < 0.05, **p < 0.01.

Article Snippet: Subsequently, we utilized 5% milk to block the membranes at 24 °C temperature and treated with anti-CD63 primary antibodies (1:1000; Abcam, ab216130), CD9 (1:1000; Proteintech, 20597-I-AP), LFNG (1:5000; Abcam, ab151699), and β-actin (1:10,000; Proteintech, 60008-I-Ig) were incubated overnight at 4 °C.

Techniques: Clinical Proteomics, Western Blot, Derivative Assay, Expressing

Journal: Stem Cells International

Article Title: hUC-MSCs Attenuate Acute Graft-Versus-Host Disease through Chi3l1 Repression of Th17 Differentiation

doi: 10.1155/2022/1052166

Figure Lengend Snippet: Primer sequences.

Article Snippet: Anti-IL-17A rabbit monoclonal primary antibody (Servicebio, GB11110) was used.

Techniques: Sequencing

Journal: Stem Cells International

Article Title: hUC-MSCs Attenuate Acute Graft-Versus-Host Disease through Chi3l1 Repression of Th17 Differentiation

doi: 10.1155/2022/1052166

Figure Lengend Snippet: sh-Chi3l1-MSCs promote IL-17A production in aGvHD mice. (a, b) Lymphocytes were obtained from the spleens of aGvHD mice on day 14 after hUC-MSC, sh-Chi3l1-MSC, sh-NC-MSC (1 × 10 6 ), or PBS (0.2 ml) treatment. Intracellular cytokines in lymphocytes were measured by flow cytometry. (a) IFN- γ and IL-17A were analyzed by flow cytometry. (b) The proportion of intracellular cytokines IFN- γ and IL-17A was measured in different groups. (c) Serum IFN- γ and IL-17A were measured by ELISA ( n = 8, three independent experiments, one-way ANOVA, and Tukey's multiple comparison test, ∗ P < 0.05; ∗∗ P < 0.01; ns: not significant). IFN- γ : interferon-gamma; IL-17A: interleukine-17A.

Article Snippet: Anti-IL-17A rabbit monoclonal primary antibody (Servicebio, GB11110) was used.

Techniques: Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Stem Cells International

Article Title: hUC-MSCs Attenuate Acute Graft-Versus-Host Disease through Chi3l1 Repression of Th17 Differentiation

doi: 10.1155/2022/1052166

Figure Lengend Snippet: sh-Chi3l1-MSCs failed to repress IL-17A production in the intestine. (a) Intestinal immunohistochemical staining results of aGvHD mice on day 21 after hUC-MSC, sh-Chi3l1-MSC, sh-NC-MSC (1 × 10 6 ), or PBS (0.2 ml) treatment. (b) Positive results of IL-17A expression measured using Image-Pro Plus software. Scale bar: 100 μ m, n = 7, three independent experiments, one-way ANOVA, and Tukey's multiple comparison test, P < 0.001.

Article Snippet: Anti-IL-17A rabbit monoclonal primary antibody (Servicebio, GB11110) was used.

Techniques: Immunohistochemical staining, Staining, Expressing, Software

Journal: Stem Cells International

Article Title: hUC-MSCs Attenuate Acute Graft-Versus-Host Disease through Chi3l1 Repression of Th17 Differentiation

doi: 10.1155/2022/1052166

Figure Lengend Snippet: Chi3l1 deletion compromises hUC-MSCs and inhibits T cell proliferation. (a–c) CD3 + T cells obtained from the spleens of healthy C57BL/6J mice were stained with CFSE (5 μ M) and then incubated in 24-well plates with hUC-MSCs, sh-Chi3l1-MSCs, and sh-NC-MSCs at a CD3 + T/hUC-MSC ratio of 20 : 1 for 72 hours. (a) Proliferation of CD3 + T cells analyzed by flow cytometry. (b) CD3 + T cell proliferation was measured after treatment with different hUC-MSCs. (c) mRNA expression levels of IFN- γ , IL-17A, and FOXP3 analyzed by qPCR (three independent experiments, one-way ANOVA, and Tukey's multiple comparison test, ∗∗ P < 0.01; ns: not significant).

Article Snippet: Anti-IL-17A rabbit monoclonal primary antibody (Servicebio, GB11110) was used.

Techniques: Staining, Incubation, Flow Cytometry, Expressing